Liquid dispensing apparatus

ABSTRACT

A liquid dispensing apparatus for use in performing the Coombs anti-globulin test. The Coombs injecting mechanism includes a removable cartridge containing the serum. The cartridge comprises a tubular barrel which is closed at its forward end by a nozzle and at its rear end by a cylindrical piston positioned within the bore of the barrel. The mechanism further includes a plunger for driving the piston into the bore of the barrel to dispense the liquid from the forward nozzle downwardly into a vial upon demand from a Coombs blood test machine associated therewith.

United States Patent [191 Genese et al.

LIQUID DISPENSING APPARATUS inventors: Joseph N. Genese, Paterson; Edward J. Rapoza; Charles F. Galanaugh, both of Butler; Harry M. Kennard, Chester; Roger A. Chevalaz, Rockaway; John A. Smith, East Orange, all of NJ.

Becton, Dickinson and Company, East Rutherford, NJ.

Filed: Feb. 27, 1973 Appl. No.: 336,280

Related US. Application Data Division of Ser. No. 160,868, July 8, 1971, Pat. No. 3,785,533.

Assignee:

US. Cl 222/37, 128/218 A, 222/4-1, 222/390 Int. Cl. G0lf 11/06 Field of Search 222/37, 41, 46,63, 390; 128/218 A; 192/141, 142 R References Cited UNITED STATES PATENTS 2/1955 Glass 128/218 A Feb. 11, 1975 12/1960 Hyde 222/37 X 3/1966 Schafer 222/390 X 5/1967 Oehme 222/390 X Jewett 128/218 A Primary E.raminer-R0bert B. Reeves Assistant Examiner-Thomas E. Kocovsky Attorney, Agent, or Firm--Kane, Dalsimer, Kane, Sullivan and Kurucz ABSTRACT A liquid dispensing apparatus for use in. performing the Coombs anti-globulin test. The Coombs injecting mechanism includes a removable cartridge containing the serum. The cartridge comprises a tubular barrel which is closed at its forward end by a nozzle and at its rear end by a cylindrical piston positioned within the bore of the barrel. The mechanism further includes a plunger for driving the piston into the bore of the barrel to dispense the liquid from the forward nozzle downwardly into a vial upon demand from a Coombs blood test machine associated therewith.

2 Claims, 12 Drawing Figures PATENTEB FEB] 1 I975 SHEET 1 BF 8 PATENTED FEB] 1 I975 SHEET 3! OF 8 PATENTED FEB] 1 i975 SHEEI SM 8 PATENTEW' 13.86527 SHEET 6 OF 8 FIG. 6

PATENTEB FEB] 1 I975 SHEET 8 OF 8 lllll Hllllllll 1 LIQUID DISPENSING APPARATUS This is a division, of application Ser. No. 160,868, filed July 8, 1971 now US. Pat. No. 3,785,533.

BACKGROUND AND SUMMARY OF THE INVENTION invention is designed to automatically perform the Coombs antiglobulin test.

In the Coombs test as presently performed, a technician initially places a sample of red blood cells to be tested in a vial. A relatively large amount of saline is then added to form a homogeneous mixture, and the vial containing this mixture is placed in a centrifuge where it is spun at approximately 1,100-1,3OO RCF (relative centrifugal force, or Gs) for about 1 minute. Centrifugation causes the red cells to be washed through the saline to form a button of cells at the bottom of the vial. Next, the saline is removed from the vial by decanting, the button of red cellsremaining at the bottom of the vial. This completes the washing cycle which is normally conducted 3 times. After the three wash cycles, approximately two drops of Coombs serum are added to the cells. This mixture is agitated by manually shaking the vial to mix the cells with the serum, and then centrifuged at approximately 1000 RCF for about seconds. To analyze the results, the

technician removes the vial from the centrifuge and vi-, sually determines whether agglutination has occurred.

If so, the test is considered to be positive, indicating incompatible antibodies on the patients red cells.

It will be apparent from the above description that the Coombs test as presently performed is a timeconsuming operation. In addition, modern blood banks require that a great number of these tests be continuously conducted to determine the compatibility of blood samples from different individuals. These factors combine to create a problem not only in obtaining the necessary number of highly trained personnel to conduct the tests, but also in insuring accurate test results. A false determination could have fatal consequences, and could easily result from an inaccurate measurement of one of the reagents or from an inexact timing of a particular operation in the sequence.

Among the several other objects and advantages of the present invention are the following:

1. The provision of an apparatus capable of controlling and standardizing the several important variables inherent in the manual Coombs test procedure, as for example, the quantity of saline used in each wash cycle, the quantity of Coombs serum added to each vial, and the centrifugation speed and time.

2. The provision of a fill system for both the saline and Coombs serum, including a photoelectric detection system whereby the fluid is automatically dispensed only intothe vials positioned in the apparatus.

3. The provision of a cartridge for both transporting FIG. 1 is an exploded perspective view of the basic components of the apparatus of the invention;

FIG. 2 is a fragmentary sectional elevation view of the head assembly portion thereof;

FIG. 3 is a fragmentary front elevation view thereof;

FIG. 4 is a top plan view thereof;

FIG. 5 is a fragmentary top plan view of a portion of the apparatus of the invention including the drive assembly;

FIG. 6 is atop plan view of the Coombs injection assembly portion of the apparatus of the invention;

FIG. 7 is a partially sectional side elevation view of the Coombs injection assembly portion;

FIG. 8 is an exploded perspective view of a cartridge utilized with the apparatus of this invention;

FIG. 9 is a sectional side elevation view thereof with the cartridge in an assembled form for use;

FIG. 10 is an end plan view of the cartridge of the apparatus of the invention;

FIG. 11 is a side elevation view of the cartridge in assembled form for use with the remainder of the apparatus; and

FIG. 12 is a side elevationview of the cartridge in condition for shipping prior to its assembly for use with the apparatus of this invention.

DESCRIPTION OF THE PREFERRED EMBODIMENT General Description of Overall Apparatus The various components of the present invention are illustrated schematically in FIG. 1. Generally, the apparatus comprises a circular turntable or head 10 mounted for rotation on a vertical shaft 12 which extends through the stationary base 14 and which is coaxially secured to the shaft 18 of the main motor 20. The head 10 isfrictionally mounted on the tapered end 12' of the shaft 12 and is secured theretoby the knurled head retaining screw 19. Thus, the head 10 rotates with the shaft 12. A conventional one-way clutch bearing 16 is frictionally mounted about the shaft 12 such that its internal race rotates with the shaft. The external race of the bearing is secured to the base 14 and thus remains stationary. By this arrangement, it will be apparent that the head 10 is free to rotate only in one direction.

The opposite end of the main motor shaft 18 mounts an engagement wheel 22. The wheel 22 is adapted to be selectively connected to an index wheel 24 across a pivoting idler 25. The index wheel 24 is connected to the drive shaft of the index motor 26.

The head assembly of the apparatus includes the head 10 which supports a plurality of trunnions 30 piv- Each trunnion also includes a plurality of individual and dispensing the Coombs serum, the cartridge being adapted to be mounted in the Coombs fill system without having to transfer the serum to a separate container first.

BRIEF DESCRIPTION OF THE DRAWINGS In the drawings:

flags 34 and is adapted to removably receive a tube carrier 36. Each carrier 36 is in turn adapted to receive a number of test tubes or vials 38. A windage bowl 40 encloses the bottom and sides of the entire head assembly.

The saline fill assembly is designated generally at 42 in the drawings and includes a discharge tube 43 leading to a nozzle 44 (FIG. 2). The saline assembly is adapted to periodically inject saline downwardly from the nozzle 44 into a tube 38 which is positioned in the tube carrier 36 on the head.

The Coombs serum injection assembly is designated generally at 46 and includes a plunger 47, a serumfilled cartridge 48 having a nozzle 49, a cartridge holding frame 50, and an injection motor 51 for driving the plunger 47. To detect the presence of a tube 38 on the rotating head of the apparatus, a photocell is mounted at 53 immediately adjacent the injection nozzles 44 and 49. The photocell is adapted to monitor the light beam 54 emanating from the bulb 55. As will be more fully described hereinafter, the presence of a tube 38 in its tube carrier causes the assorted flag 34 to interrupt the light beam 54 as the head is rotated past the holding frame 50 for the saline and Coombs injection nozzles. The interruption of the light beam actuates either the saline fill system or the Coombs injection system.

The outer cabinet of the apparatus is generally indi cated at 60 in FIG. 3. The cabinet includes a rectangular box-like framework which is covered by suitable panels to enclose the working components of the machine. The upper portion of the cabinet includes a sliding glass or plastic lid 61 which is designed to permit entry to the head and injection assemblies when opened, and to protect the technician when closed during the operation of the device. If desired, a suitable switch (not shown) may be provided whereby the machine will operate only when the lid is closed.

A control panel 62 is mounted on the front of the cabinet as best seen in FIG. 3. The control panel includes an on-off switch 63 which controls the power to the machine. A green power indicator light is mounted at 64 and is lighted whenever the power switch is on. A start button and indicator light'is mounted at 65 which, when actuated, starts the automatic cycle and lights green when the cycle is in progress. A stop button and red indicator light 66stops the machine at any time during thecycle and lights red when themachine is stopped or the lid is open. A 500 RCF spin button 67 starts an independent intermediate spin as will be described hereinafter. A low indicator light is mounted at 68 and lights amber when the Coombs cartridge 48 is low on serum. An empty indicator light is mounted at 69 and lights red when the Coombs cartridge is empty. Fill, spin, and decant indicator lights are mounted at 70, 71 and 72, respectively. These lights are lighted when these points in the-automatic wash cycle are reached. The programmer 73 is connected to the main timer of the apparatus and indicates which stage has been reached in the cycle. It can be turned to select any of the three wash cycles.

The entire head assembly is surrounded by a windage bowl 40 which may befabricated from any suitable plastic or similar material. The lower wall of the bowl includes an aperture 108 having a glass lens mounted at 109. From FIG. 2, it will be apparent that the aperture 108 is designed to permit the light beam 54 emanating from the bulb 55 to reach the photocell at 53. The bowl 40 further includes a drain (not shown) positioned at the intersection of the bottom and side walls.

HEAD ASSEMBLY 4 axially secured to the rotor 18 of the reversible main motor 20. The shaft 12 extends through the fixed base member 14 and includes a tapered end 12'to receive a correspondingly tapered bore in the hub of the head 10. A knurled head retaining screw 19 is coaxially inserted into the shaft 12 to retain the positioning of the head on the shaft 12, note FIG. 3.

The one-wayclutch bearing 16 has its internal bearing surface frictionally engaged by the shaft 12, and its external surface is secured to the fixed base 14. By this arrangement, the head may be rotated only in one direction, that being counterclockwise in the illustrated embodiment. This feature is utilized in conjunction with the braking operation which occurs at the termination of the centrifuging operations. In particular, reversal of the polarity of the motor 20 while the head is rapidly rotating creates a resistance to continued rotation. When the head completely stops, rotation in the opposite direction is precluded by the bearing 16.

The outer periphery of the head 10 includes four trunnion mounting stations, each defined by a pair of outwardly extending parallel lugs 75. The two lugs at each station include aligned openings 76 (FIG. 1) therethrough which define a chord line along the periphery of the head. The openings 76 are adapted to receive the mounting pins 31 for the trunnion 30, whereby the trunnion is rotatablymounted about the axis formed between the two openings 76. I

DRIVE ASSEMBLY The drive assembly'for the head 10 is illustrated in detail in FIGS. 3 and 5. This assembly includes the reversible motor 20 which drives the head l0 during the centrifuging and decanting operations and the index motor 26 which drives the head during the saline and Coombs injection operations.

The lower end of the rotor 18 to the motor 20 mounts the engagement wheel 22. Interposed between the motor 20 and 'wheel 22, is a conventional centrifugal spaced control governor 110 for the motor, the function of which will be mentioned hereinafter. The wheel 22 permits the head 10 to be operatively connected to the index 'motor' 26 across the index wheel 24 and idler 25 during the saline fill andCoombs injection operations. In the disclosed embodiment, the motor 26 is designed to rotate at approximately 1 RPM and, by reason of the relative sizes of the wheels 22 and 24, it drives the head 10 at a slightly greater speed.

To control the injection systems during indexing of the head 10, a series of cam operated switches 112-1 14 are positioned about the periphery of the index wheel 24. These switches are adapted to ride on the coaxially disposed cam wheel 116 and be actuated upon engagement with the cam 117. It will be noted that the index wheel 24 also has a cam 118 on its outer periphery, the cam 118 being adapted to disengage the idler 25 from the engagement wheel 22.

At the initiation of the indexing operation, the index wheel cam 118 will have lifted the idler 25 from the engagement wheel 22 to the position shown in dotted lines, and the switch 112 will be tripped'by the cam 1 17. The switch 112 acts as a safety feature in that centrifugation can only occur if it is tripped (indicating that the idler 25 is disengaged from the wheel 22), and thus there is no chance that the wheel 24 will be rotated during contrifugation. During initial rotation of the index wheel 24 in a clockwise direction as seen in FIG.

5, the idler will be pulled into operative engagement with the two wheels by the spring 120 which is connected to the fixed frame member 122. The idler 25 preferably comprises two coaxially mounted hard rubber wheels to insure maximum frictional engagement.

After rotation of approximately the cam 117 engages the switch 113 which arms both the saline fill and Coombs injection systems. Since there may well be some initial slippage across the rubber idler wheels, a 30 idle is built into the system by this arrangement.

After arming of the injection systems, the photoelectric system begins to search for a horizontal flag indicating the presence of a vial 38 in the carrier 36. When the light beam 54 is interrupted by the presence of a horizontal flag arm, the index motor 26 is stopped and the appropriate injection cycle actuated. Upon completion of the injection, the index motor is started to continue the operation. This searching operation continues until the wheel 22 has completed a 360 revolution, at which time the cam 117 actuates the injection disarming switch 114. By design, the circumferential distance along the wheel 24 between the-switches 113 and 114 is exactly equal to the circumference of the engagement wheel 22. Thus the injection systems are armed for exactly one revolution of the head 10. After disarming, the index wheel 24 continues to rotate until the switch 112 is again tripped by the cam 117 to enable subsequent centrifugation. At this point, the idler 25 is lifted from the engagement wheel 22 by the cam 118 such that the index wheel 24 will not be rotated during the subsequent centrifugation and decanting operations.

COOMBS INJECTION ASSEMBLY The Coombs injection assembly 46 is illustrated in FIGS. 6-11 and includes the cartridge 48 which contains a measured amount of the serum. Typically, the cartridge contains 9 ml (milliliters) of the serum, which is sufficient for 100 tests, i.e.-, 0.09 ml per test. The cartridge 48 is designed to be disposable after use,.and serves not only asthe transfer vessel for the serum from the manufacturer, but also as an accurate dispensing mechanism when it is coupled with the remaining portion of the injection assembly 46.

As best seen in FIGS. 8-11 the cartridge 48 com prises a tubular glass barrel 150 having a dimensionally controlled internal diameter. The external surface may, if desired, be graduated as at 151 to indicate the number of doses remaining. The forward end of the barrel includes a threaded neck portion 152 which is designed to be sealed during shipment by a separate closure cap 153 (FIG. 12) in the conventional manner. The rear end of the barrel is closed by a hard rubber cylindrical piston 154 which may include a circumferential resilient rubber sealing ring 155.

To prepare the cartridge for use, the closure cap 153 is removed and the nozzle 49 inserted ,into the open neck of the barrel. The nozzle 49 may be fabricated from a similar hard rubber material, and includes an enlarged diameter plug portion 157 which is somewhat less in diameter than the internal diameter of the barrel neck 152. The plug portion 157 of the nozzle is designed to take up the ullage in the cartridge and thereby prevents the subsequent ejection of air. Intermediate the ends of the nozzle is a circular flange 158 which is adapted to overlie the open neck end of the barrel, see FIG. 18. When the cap 160, which has an opening through its bottom wall to receive the outer portion of the nozzle, is tightly threaded onto the neck, the flange 158 provides a sealing engagement between the two members. The forward end of the nozzle- 49 includes a flattened guide portion 161 which is adapted to facilitate mounting of the cartridge as will thereinafter be further described.

It will be appreciated that the nozzle 49 includes a small diameter bore 162 therethrough which terminates in the conically shaped nipple 163. The conical shape of the nipple. tends to reduce the tendency of the serum to form a droplet at the discharge end. Since a very small amount of serum is used in each test, the formation of such a droplet could significantly affect the amount of serum being discharged.

The sealed cap is used for storage and shipment of the cartridge, while the nozzle is used when injection of the serum is to be performed. Other external features of the cartridge are apparent. One is that it has graduations on its exterior which provide a continuous measure of the number of drops of serum remaining in the cartridge. Another external feature is that the nozzle is provided with a key precisely made to properly position the cartridge in the Coombs machine when the key is engaged in the machines keyway. The operation and structure of these features are readily apparent from the description of the cartridge included herein. This is particularly true in regard to the proper positioning of the cartridge within the remainder of the apparatus.

It should also be kept in mind that all of the surfaces which contact the serum are constructed of material which has demonstrated compatibility with the serum. Furthermore, the nozzle incorporates a cylindrical section to take up space in the neck portion of the glass cylinder so that no serum is wasted when the piston is in the empty position. Additionally, the nozzle and plunger are shaped to permit priming of the cartridge prior to use and to minimize unusable serum. Also, it should be noted that the nozzle exit is angled slightly with respect to a perpendicular with the cartridge center line. Hence, when the cartridge is mounted in the machine with its center line horizontal, the nozzle injection stream is not vertical. This feature, together with the use of a proper internal pressure in the cartridge, is used to shoot" the serum at a nonvertical angle. In this way, a number of injections may be injected into the same tube if it is so desired.

The sealing ring provided on the piston also provides a specific function in addition to its normal use of providing a seal. The additional function of note is to compensate for temperature (differential expansion) effects over the operating temperature range. This is important for if the friction were allowed to vary greatly, the internal pressure generated by the piston would vary and, in addition, a piston positioned-sensing switch in the machine could be adversely affected.

It should also be noted that the sealing ring is a Quad (quadrangular) ring rather than an O-ring to provide a better zero pressure seal. Furthermore by providing the sealing ring on a reduced forward portion of the plunger, the seal area is free from parting lines and sealing is accomplished on smoother surfaces.

Also, by providing pressure onthe sealing in one direction only, when the plunger motion is reversed the Quad ring tends to come off the plunger. This will reduce the tendency to rinse the cartridges which is undesirable since they are disposable and should not be reversed. v

Additionally, utilizing the Quad ring rather than an O-ring, provides better low ambient pressure resistance. Since the Quad will not roll like an O-ring, it resists backward motion which can be caused by elevated temperatures and/or exposure to high altitude pressure. (In both instances the pressure inside the cartridges will exceed the external pressure.)

It is readily apparent from the description of the cartridge that it will serve a dual function. It is a container for shipment of serum, within which serum is stored and shipped, and then later becomes an automatic dispenser for the contents. The cartridge assembly is therefore a convenient vehicle which minimizes unnecessary handling or providing an accurate means for dispensing its contents.

To complete the description of the Coombs assembly 46, reference is made to FIGS. 6-7. The assembly is mounted on a fixed frame 166 which includes a front horizontal holder or platform. .The forward end of the holder mounts both the nozzle 44 of the saline assembly 42 and the cartridge 48 as best seen in FIGS. 2 and portion 161 of the nozzle 49 and thereby prevent lat-' eral and rotational movement of the cartridge. Also, a longitudinal channel 171 in the holder is designed to partially receive the cartridge and thereby to facilitate retention of the cartridge in its proper position.

A second opening 172 through the forward end of the channel is positioned radially inwardly from the opening 168. This second opening 172 is designed to mount a photocell53 in a suitable retainer which is re ceived in the channel'171. By design, the photocell 53 is positioned to monitor the light beam .54 emanating from the bulb 55.

To discharge the serum from the cartridge, the plunger 47 is mounted for axial movement against the rear wall of the piston. Thus movement of the plunger 47 drives the piston 154 into the barrel 150 to forcibly eject the serum through the nozzle 49 and into the underlying via] 38. The forward portion of the plunger is externally threaded, with the threads being engaged by the drive nut 175. The nut, which is rotatably driven by the gear 176, is keyed to the frame to prevent its axial movement. Thus, rotation of the nut 175 causes the plunger 47 to either advance or retract.

The gear l76 meshes with the gear 177 which is connected to the injection motor 51. By this arrangement, rotation of the motor causes the rotation of the gears 176 and 177 and nut 175, and thus the advance of the plunger 47. Rotation of the motor 51 in the opposite direction will cause the plunger to retract.

To accurately control the forward movement of the plunger, and thus the amount of serum ejected from the nozzle 49, a pair of cams 180 and 181 are mounted for rotation with the gear 176 and nut 175. In the particular embodiment of the assembly described herein, the gear and nut are designed to rotate one quarter turn per dosage of Coombs serum. Thus a stop signal to the motor- 51 is required after the plunger has been advanced by a quarter turn of the nut. To provide this function, the forward cam includes four equally spaced bumps which are designed to actuate the switch 183. The second coaxially mounted four bump cam 181 is positioned to actuate the override switch 184 for the purpose described in the following paragraph.

Describing the sequence of operations during the Coombs injection mode, the turntable is slowly rotated by the index motor 26. Whenever the photocell 53 senses a horizontal flag, a signal is sent by conventional circuitry from the photocell circuit to stop the index motor 26 and start the Coombs injection motor 51 which rotates the nut 175 to advance the plunger 47. Rotation of the nut 175 for a quarter turn discharges the desired amount of serum into the underlying vial 38, at which time the switch 183 stops the motor 51. Concurrently, the switch 184 overrides the. photocell circuit to reactivate the index motor 26. This cycle is repeated whenever a vial is present on the turntable.

The rear portion of the Coombs assembly includes a fixed horizontal platform 186 having a slot 187 running immediately below the rear portion of the plunger. The

plunger includes a downwardly extending fin 188 which is-adapted to ride in the slot to thereby prevent the rotation of the plunger.

The opposite sides of the rear portion of the plunger include cam surfaces 190 and 191 which'terminate in abutments 192 and 193 respectively, note FIG. 6. A switch 194 is associated with the surface 190 and is actuated when its arm engages the abutment 192. Similarly, the switch 195 is actuated when its arm engages abutment 193. It will thus be apparent that as the plunger advances into the cartridge, switch 194 will first-be actuated and switch 195'will subsequently be actuated. By design, these switches indicates when the cartridge is low on serum (e.g., less than 25 doses remaining) and when the cartridge is empty, respectively. The switch 194 controls the low signal lamp 68 on the controlpanel and the switch 195 controls the empty signal la'mp 69.

After the cartridge has been initially placed on the assembly 46, it is desirable that the plunger be rapidly advanced until it engages the rear wall of the cartridge piston 154. Also, it is desirable that an automatic stop for the plunger advance be provided when contact with the piston is made. For this purpose, a rapid advance switch (not shown) is placed on the cabinet of the apparatus to independently actuate the injection motor 51 and thereby advance the plunger, the advance in this case being independent of the stop switch 183. In addition, a push rod 198 is positioned in a central bore extending through the length of the plunger 47. As indicated in FIG. 16, the rod 198 is normally biased to protrude a short distance ahead of the forward end of the plunger. A switch 199, which is mounted to ride on the rear end of the plunger, is actuated whenever the rod 198 is depressed into the plunger. Thus during operation of the rapid advance, the plunger advances until the rod contacts the rear wall of the piston 154. By design, the switch 199 is actuated just as the forward tip of the plunger 47 comes into engagement with the wall of the piston. The switch 199 overrides the rapid ad,

vance switch and terminates rotation of the motor 51. For rapid retraction of the plunger, a retraction switch (not shown) is also placed on the cabinet to independently actuate the injection motor 51 in the reverse direction. A switch 200, which is mounted to the fixed platform 186, is adapted to be actuated by the plunger when it reaches its point of maximum retraction. Switch 200 similarly overrides the retraction switch to stop the injection motor 51.

Also associated with the Coombs injection system is a priming switch (not shown) which is mounted on the external cabinet of the apparatus. The priming switch, when actuated, cycles the injection system once and thus is effective to discharge one dose of serum from the nozzle. By cycling the system 1 or 2 times after the plunger 47 has contacted the piston 154, the technician will have complete assurance that no air is in the system and that subsequent ejections will contain the proper amount of serum.

OPERATION OF THE APPARATUS To initially prepare the apparatus of the present invention to run a Coombs test, the saline supply container is first connected to the tube 125 leading to the saline fill apparatus. The saline fill system should then be primed by actuation of the priming switch a number of times until all of the air is expelled.

Next, the drainage port in the windage bowl 40 is connected via the external connection 204 to a catch bottle (not shown). Since drainage the bowl depends on gravity, the catch bottle should be positioned below the windage bowl.

The cartridge 48 is next positioned on the'holder 50 in the manner shown in FIGS. 6-7. To rapidly advance the plunger into contact with the cartridge piston, the rapid advance switch is closed to energize the motor 51 and rotate the nut 175. When the contact with the piston 154 is made, the push rod 198 actuates switch 199 to terminate the advance operation before any serum is dispensed. It will be seen that this arrangement facilitates the use of partially filled or previously used cartridges. Thus whether the cartridge 48 is completely full or not, the plunger may be quickly brought into engagement with the piston 154. To prime the system, the priming switch is actuated a number of times until no air is discharged from the nozzle.

While the head 10 may be removed from the shaft 12 by removing the knurled head retaining screw 19, it will be assumed that the head is properly positioned on the shaft and that the screw 19 is securely hand tightened.

The apparatus is now ready for operation. To perform each test, a measured amount of red blood cells is first manually placed in a vial 38. This vial, together with up to five other vials, may be placed in a single carrier. Since the apparatus described he rein is adapted to receive four carriers, it will be apparent that up to 24 tests may be conducted simultaneously on each run of the machine. In the interest of maintaining balance of the device during centrifugation, it is recommended that only an even number of vials be placed on the head, with the vials being evenly spaced about the periphery.

With the on-off switch 63 in the on" position, the programer 73 (FIG. 3) is turned to a point at the start of the first wash cycle. The start button 65 is then actuated to start the automatic cycle. While the programmer 73 has not been described in detail herein, it will be appreciated that it is of a conventional and well known design, and that it is operative to sequentially initiate and terminate the various operations of the apparatus. A programer of this type may be purchased from the Cramer Division of the Conrac Corporation, Elmsford, New York.

The programer 73 initially actuates the saline fill cycle. During this cycle, the head 10 is slowly rotated in a counterclockwise direction by the index motor 26. After actuation of the arming switch 113 by the cam 117, each vial will automatically receive approximately 3 ml of saline as it passes under the discharge nozzle 44. More particularly, the vial 38 will have rotated the associated flag to its horizontal position. As the flag breaks the light beam 54, the photoelectric circuit acts to stop the motor 26 and actuate the solenoid 136 to discharge the proper amount of saline into the vial. By reason of this unique sensing mechanism, only those carrier positions with vials present will receive saline. When the solenoid reaches the top of its stroke, the switch 138 is actuated which releases the solenoid and overrides the stop signal from the photocell circuit to again start the index motor 26. It will be noted from FIG. 2 that the saline is forcibly ejected from the nozzle 44 against the side of the vial. In view of this fact, the entering saline will swirl and completely mix with the blood cells in the vial.

When the fill cycle has been completed, the programer 73 initiates the spin cycle. In the present embodiment, the head 10 is rotated for approximately 93 seconds; l8 seconds to reach speed, 60 seconds at 3200 RPM (approximately I300 RCF) and 15 seconds for braking to stop.

During centrifugation, the trunnions 30 are rotated to the extending position C shown in FIG. 2 to assure retention of the carriers and vials, as well as the contents of the vials. As previously noted, braking is accomplished by reversing the polarity of the motor 20.

When rotation of the head 10 stops, the electromagnetic coils beneath the base I4 are actuated to pull the magnetic inserts 32 on the trunnions toward the shaft 12 to position A. The usual angle of centrifugation of the vials is thus reversed. The centifuge now operates for 3 seconds at approximately 290 RPM, and due to the reverse negative angle of the vials, the saline is thrown upwards and out of the tube vials. However, the red blood cells remain. It will be appreciated that the particular negative angle of decant employed, as well as the speed and time of rotation, must be closely interrelated to assure retention of a button of red blood cells in the vials.

The above described wash cycle, comprising the saline injection, spin and decanting, occurs automatically 3 times. At the termination of the third wash cycle, the programer 73 initiates the Coombs injection cycle. As in the wash cycle, the turntable 10 is slowly rotated by the index motor 26. Whenever the photocell 53 senses a horizontal flag, a signal is sent to stop the index motor 26 and start the'Coombs injection motor 51. The motor 51 rotates the nut for a quarter turn to discharge the desired amount of serum into the underlying viall At this point, the switch 183 stops the motor 51 and the switch 184 overrides the photocell circuit to reactivate.

the index motor 26. This cycle is repeated whenever a vial is present on the turntable as the turntable rotates through one complete revolution during which the system has been armed by the switch 113 at the index wheel 24.

Both immediately before and when the Coombs fill cycle has been completed, the electromagnetic coil 105 1 1 is pulsated such that the trunnions 30 and vials 38 are alternately drawn to the pole ring 33 and then flipped back by the springs 90 and 91. This operation insures complete mixing of the cells with the Coombs serum as well as proper agitation before addition of the Coombs serum.

Next, the head is centrifuged for an additional 90 seconds; 18 seconds to reach speed, 60 seconds at 1900 RPM (500 RCF) and seconds braking to a stop. The entire test cycle is then completed, and the final fluid in the vial is ready for subjective analysis by the technician.

It will be recalled that the control panel 62 on the front of the apparatus includes a pushbutton 67 designated 500 RCF spin. Actuation of this button initiates a 500 RCF spin cycle which is independent of the programer,73. This feature permits the apparatus to be used for other blood bank centrifugation purposes, the cycle of which is the same as the 500 RCF spin cycle in the automatic Coombs procedure. In particular, thecentrifuge will operate for 83 seconds; 18 seconds to reach speed, 60 seconds at 1900 RPM (500 RCF), followed by 5 seconds braking to stop.

lt will also be apparent that in order to accurately perform the Coombs test, suitable controls must be provided to accurately govern the rotational speed of the main motor 20. In this regard, it will be recalled that the motor initially rotates the head at a speed of approximately 3200 RPM during the wash-spin cycle, at 1900 RPM during the Coombs spin cycle (as well as Y the independent 500 RCF cycle), and at 290 RPMdurcartridge comprising a tubular barrel closed at one end by a nozzle and at the opposite end by a cylindrical piston, said cylindrical piston being positioned within the bore of said tubular barrel and having an external diameteradapted to sealingly engage the interior cylindrical surface of said bore, a holding frame for mounting said cartridge with said nozzle directed downwardly, same frame further mounting an axially movable plunger having a forward end adapted to engage the outer wall of said cylindrical piston, and means for axially advancing said plunger a predetermined distance whereby said piston is axially ad; vanced into the bore of said tubular barrel and a predetermined quantity of said liquid is ejected through said nozzle, means for initially advancing said plunger into engagement with the outer wall of said cylinder plug, said initial advance means comprising a control rod disposed coaxially within said plunger, said control rod being axially movable within said plunger and being normally disposed to extend a short distance beyond said forward end of said plunger, switch means at the rear end of said plunger operatively connected to said control rod to detect rearward movement of said control rod inrelation to said plunger, and said switch means acting to terminate said initial advance means when said control rod contacts the outside wall of said piston. 2. The liquid dispensing apparatus as defined in claim 1 further including means to indicate the amount of liquid remaining in said cartridge, said indicator means comprising an abutment surface positioned on said plunger intermediate the ends thereof and a cam follower mounted on said'frame and adapted to be contacted by said abutment surface during the advance of said plunger into said tubular barrel, said cam follower being operatively connected to an external signal means.

i l k i 

1. A liquid dispensing apparatus comprising: a cartridge adapted to retain a quantity of liquid, said cartridge comprising a tubular barrel closed at one end by a nozzle and at the opposite end by a cylindrical piston, said cylindrical piston being positioned within the bore of said tubular barrel and having an external diameteradapted to sealingly engage the interior cylindrical surface of said bore, a holding frame for mounting said cartridge with said nozzle directed downwardly, same frame further mounting an axially movable plunger having a forward end adapted to engage the outer wall of said cylindrical piston, and means for axially advancing said plunger a predetermined distance whereby said piston is axially advanced into the bore of said tubular barrel and a predetermined quantity of said liquid is ejected through said nozzle, means for initially advancing said plunger into engagement with the outer wall of said cylinder plug, said initial advance means comprising a control rod disposed coaxially within said plunger, said control rod being axially movable within said plunger and being normally disposed to extend a short distance beyond said forward end of said plunger, switch means at the rear end of said plunger operatively connected to said control rod to detect rearward movement of said control rod in relation to said plunger, and said switch means acting to terminate said initial advance means when said control rod contacts the outside wall of said piston.
 2. The liquid dispensing apparatus as defined in claim 1 further including means to indicate the amount of liquid remaining in said cartridge, sAid indicator means comprising an abutment surface positioned on said plunger intermediate the ends thereof and a cam follower mounted on said frame and adapted to be contacted by said abutment surface during the advance of said plunger into said tubular barrel, said cam follower being operatively connected to an external signal means. 